March 9, 2018

Our paper "Nerolidol production in agroinfiltrated tobacco: Impact of protein stability and membrane targeting of strawberry (Fragraria ananassa) NEROLIDOL SYNTHASE1" published in Plant Science

Paola Andrade, David Manzano, Karla Ramirez-Estrada, Daniel Caudepon,
Montserrat Arro, Albert Ferrer, Michael A. Phillips⁎

Plant Science 267 (2018) 112-123

Here is a link to the PDF

In this paper, Paola generated a number of transformation constructs for tobacco agroinfiltration in which the strawberry nerolidol synthase gene was fused to various targeting peptides for expression at the endoplasmic reticulum or in the cytosol. Using a quantitative volatile analysis technique, which we validate in this paper, we assess the effects of subcellular localization of a terpene synthase on sesquiterpene production. The upshot: the endoplasmic reticulum is a slightly better environment for access to the high demand substrate farnesyl diphosphate, but nothing really beats a highly stable and soluble recombinant protein. We achieved better results (nerolidol production in infiltrated tobacco leaf tissue) but simply fusing nerolidol synthase to a highly soluble partner like GFP.

Congratulations to Paola on her publication and best of luck to her at the state biotechnology laboratory at the Institute for Agricultural Research (Instituto de Investigaciones Agropecuarias) in La Platina, Chile. May there be many more.

September 11, 2017

Back to school: End of summer group photo

A group photo of the summer students working in my lab. From left to right: Ibadat Bajwa, Matthew Bergman, Alex Chang, Michael Phillips, Ekaterina Kibkalo, Riya Bali, and Haniya Adnan.

Thanks to everyone for their hard work and dedication to basic plant research this summer. Now it's back to school, but the research continues.

SmartiGras 2017: A well attended student conference in the summertime

On Aug 16, undergraduate students Ibadat Bajwa and Haniya Adnan presented their summer research at the SmartiGras undergraduate research conference. Haniya presented our new project on coriander volatile analysis and transcriptomics and Ibadat explained his project on untargeted metabolite analysis in heat and light stressed Arabidopsis plants. Good job to Ibadat and Haniya for representing the Phillips lab at this UTM sponsored event.

August 16, 2017

ROP position available in auxin biosynthesis in primitive plants

An ROP research position is currently available in my lab for a motivated undergraduate UTM student interested in plant metabolism. This project deals with the origin of auxin biosynthesis in land plants. To study this, we are looking at the Charophyte Chara vulgaris, the modern day descendant of the earliest known line which gave rise to land plants. As a Charophyte algae, Chara is an aquatic primitive plant, and there is some controversy as to how it makes auxin phytohormones. It is also unclear as to whether land plants acquired this ability from Charophytes or evolved this ability independently later on during plant evolution.

For these reasons, Chara is a useful system to study this question. In general, the origin of phytohormones in plants is a fundamental area of plant biology research, and this project holds great potential for producing significant discoveries.

What this project will involve for students: you will learn cultivation techniques for maintaining aquatic plants, molecular techniques for measuring transcript abundance by quantitative PCR, and organic extraction techniques for preparing samples for analysis. This is a student project focused on benchwork, but you must also be good at record keeping, be a detail oriented person, and have good communication skills in order to effectively present experimental data at lab meeting and conferences.

Interested students should contact me at

Starting dates: This project is scheduled to begin in the winter 2018 semester. Alternatively, if you still have space in your schedule, you can also begin this fall.

Chara vulgaris

August 15, 2017

Undergraduate research: Come visit the 2017 SmartiGras Thursday, Aug 16 on the UTM campus

The Research Opportunity Program is UTM's most successful and popular undergraduate career development program. It places undergraduates in a research environment for a semester and gives them first hand experience in laboratory research, project management, and practical training for a scientific career in academia or industry.

This Thursday, UTM undergraduates will showcase their summer research projects at the annual SmartiGras, a one day conference with talks and posters. The subjects are broad and reflective of the diverse research at UTM, and Biology is especially well represented.

Two members from the Phillips lab, Ibadat Bajwa and Haniya Adnan, will present posters on their summer projects. If you are around this Thursday and want to see what motivated undergrads can do with their summers, come check out the SmartiGras in the Instructional Building on the UTM campus this Thursday

Good luck Ibadat and Haniya!

July 13, 2017

Undergraduate Summer Research in the Phillips lab

Summer research is in full swing in the Phillips lab. Thanks to the University of Toronto - Mississauga Research Opportunity Program, four UTM undergrads are acquiring practical research experience in my lab this summer. Each student is pursuing a fully independent research project in plant biology as part of the BIO299 summer research program. Here is a summary of their projects:

Alex Chang. Alex is learning bioinformatics techniques in a collaborative project with Prof. Adriano Senatore. Alex is assembling de novo transcriptomes for essential oil plants under study in the lab to identify new genes involved in essential oil biosynthesis.

Haniya Adnan. Haniya is engaged in the molecular cloning of essential oil genes from coriander using bioinformatics and homology based cloning approaches. In addition, she is conducting volatile metabolite profiling of commercial coriander cultivars.

Ibadat Bajwa. Ibadat is applying metabolomics techniques to the study of plant responses to environmental stresses using Arabidopsis. He has optimized an untargeted sample prep protocol for the analysis of primary metabolites by GC-MS.

Riya Bali. Riya is investigating the auxin biosynthetic pathway in the charophyte alga Chara. Using a combination of isotopic labeling, gene expression analysis, and LCMS/MS targeted metabolomics, she is attempting to resolve a long standing issue in the evolution of phytohormone evolution: where and when did plants evolve the ability to make and use auxins as growth regulators?

Matthew Bergman. A recent graduate in Biology at UTM, Matthew will soon begin his PhD program in the Phillips lab. This summer, he is investigating the biosynthesis of terpenoid essential oils in rose geranium (Pelargonium sp.) using whole plant labeling, molecular cloning, and volatile analysis.

Ransher Nain. Ransher is continuing the exploration of the origin of pyruvate in plant chloroplasts. Arabidopsis genetics, whole plant isotopic labeling, and LCMS/MS analysis of primary metabolites constitute his primary research activities in the lab.

Lucy Chen. As an outstanding lab volunteer, Lucy has maintained the Arabidopsis cultures, learned genetic screening of transgenic plants, and organized the lab during the startup period.

April 20, 2017

NSERC Discovery granted awarded to the Phillips lab

The Natural Sciences and Engineering Research Council of Canada has awarded the Phillips lab a 5-year Discovery grant. The title of my proposal is "Control of Flux and Adaptive Responses at the Interface of Primary and Secondary Plant Metabolism". This grant is the basic laboratory operational fund in Canadian natural science research. It represents an important step in establishing a well funded laboratory operation and opens the door to other NSERC funding opportunities. My thanks to the anonymous reviewers and colleagues who provided valuable feedback, especially Profs. Reuben Peters, Claudia Vickers and Steven Short.